重组仓鼠卵巢细胞中OX2促食欲素/下丘脑泌素受体信号转导的研究( OX2 orexin/hypocretin receptor signal transduction in recombinant Chinese hamster ovary cells )

Kukkonen P Jyrki Orexin Hypocretin Phospholipase Calcium Adenylyl cyclase 2-AG, 2-arachidonoylglycerol AA, arachidonic acid AC, adenylyl cyclase CHO cells, Chinese hamster ovary (K1) cells CTx, cholera toxin DAG, diacylglycerol DAGL, DAG lipase dyngo 4a, 3-hydroxy-N′-([2,4,5-trihydroxyphenyl]methylidene)naphthalene-2-carbohydrazide GF109203X, (bisindolylmaleimide I Gö6850) 2-(1-[3-dimethylaminopropyl]-1H-indol-3-yl)-3-(1H-indol-3-yl)-maleimide HBM, HEPES-buffered medium HEK293 cells, human embryonic kidney cells IBMX, 3-isobutyl-1-methylxanthine IP3, inositol-1,4,5-trisphosphate nHill, Hill/co-operativity coefficient OX1 and OX2 (receptors), OX1 and OX2 orexin receptors PLA2, PLC and PLD phospholipase A2, C and D respectively PLD1i, PLD1 inhibitor (N-[2-[4-(5-chloro-2,3-dihydro-2-oxo-1H-benzimidazol-1-yl)-1-piperidinyl]-1-methylethyl]-2-naphthalenecarboxamide) PTx, pertussis toxin S-BSA, stripped bovine serum albumin TCS 1102, N-biphenyl-2-yl-1-{[(1-methyl-1H-benzimidazol-2-yl)sulfanyl]acetyl}-l-prolinamide

我们研究了中国仓鼠卵巢细胞OX2orexin受体的信号转导，并与OX1receptor的信号转导进行了比较，发现OX1receptor的信号转导包括不同的磷酸脂酶、Ca2+和腺苷酸环化酶，结果表明OX2orexin受体的信号转导与腺苷酸环化酶的信号转导是相似的，但磷脂酶D和Gs的信号转导存在一些差异，有待于进一步研究。

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