芦荟素合成纳米银粒子对实验性小鼠乳腺炎模型治疗潜力的评估。( Evaluation of therapeutic potential of nanosilver particles synthesised using aloin in experimental murine mastitis model. )

None biochemistry cellular biophysics diseases drugs microorganisms molecular biophysics nanofabrication nanoparticles organic compounds patient treatment proteins reduction (chemical) nanomedicine therapeutic potential evaluation murine mastitis model nanobiotechnology aloin mediated nanosilver particle synthesis silver nitrate reduction Staphylococcus aureus broth culture S aureus ketamine anaesthesia blunt Hamilton needle post inoculation intramammary routes intraperitoneal routes antibiotic cefepime serum C reactive protein mammary gland bacterial load reduced glutathione content thiobarbituric acid reactive substance content superoxide dismutase activity catalase activity histopathology polymorpho nuclear cell infiltration in vitro spleenocyte viability time 24 h time 6 h time 18 h Ag

纳米生物技术是纳米技术的一个新兴生物学分支。具有特定尺寸和形状的纳米粒子在生物学中的应用已经显示出意想不到的有趣结果。进行了一项研究，以评估通过用芦荟素还原硝酸银制备的植物源性芦荟素介导的纳米银颗粒（AAgNPs）对金黄色葡萄球菌诱导的鼠乳腺炎的治疗潜力。将总共40只雌性小鼠分成5组，每组8只动物。I组作为泌乳对照组，II-V组在氯胺酮麻醉下接种20l含4.0×105cfu/季度的金黄色葡萄球菌24h肉汤培养物。接种后6小时，III组和IV组分别通过乳房内和腹膜内途径接受20l芦荟素纳米银（AAgNPs）。V组经腹膜内途径给予抗生素头孢吡肟1mg/kg体重。治疗后18h测定血清C反应蛋白、乳腺重量、乳腺细菌载量、硫代巴比妥酸反应物质含量、还原型谷胱甘肽含量、超氧化物歧化酶活性和过氧化氢酶活性及组织病理学。该化合物对金黄色葡萄球菌的最小抑制浓度为21.8 ng/ml。与抗生素（50%）相比，观察到AAgNPs显著减少（98%）多形态核细胞浸润。

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